Instruments

ELECTRON MICROSCOPES (and ancillary support equipment)

  • FEI Talos 200SC FEG TEM equipped with Ceta and Falcon II Direct Detection CMOS cameras (NIH SIG award # 1S10RR023454-01, McCaffery PI)
  • FEI Tecnai 12 TWIN TEM equipped with 16 bit 2K x2K FEI Eagle bottom mount camera; and SIS MegaView III wide-angle camera (acquired with funds from NSF DBI award # 0099706, McCaffery PI and NIH SIG award # 1S10OD012342-01A1, McCaffery PI)
  • Philips EM 420 TEM equipped with EMSIS MegaView G3 CCD digital camera (acquired in part with funds from NIH R01GM060979-06, Wendland PI)
  • FEI Quanta 200 Environmental SEM (acquired with funds from HHMI undergraduate education grant)
  • FEI Vitrobot vitrification robot (acquired with funds contributed by WSE and KSAS)
  • Tousimis 795 Critical Point Dryer
  • Anatech Hummer 6.02 Sputter Coater
  • Denton 502A High Vacuum Evaporator
  • Two Leica Ultracut UCT Ultramicrotomes/FCS Cryostages (acquired in part with funds from NSF DBI award # 0099706, McCaffery PI)
  • LaVision BioTec UltraMicroscope II light sheet microscope equipped with 405, 488, 561, 640, and 785 nm lasers and Okolab incubator (acquired with funds from NIH SIG award #1S10OD020152-01A1, McCaffery PI)
  • Two Zeiss LSM 700 confocals (Left and Right) equipped with 405, 488, 555, and 633 nm lasers and PeCon cage incubators for live imaging
  • Zeiss LSM 780 confocal microscope with PeCon cage incubator
  • Zeiss LSM 800 confocal with Airyscan super-resolution module, equipped with 405, 488, 561, and 633 nm lasers, motorized stages, and PeCon incubator(acquired with funds from NIH SIG award #1S10 OD020152-01A1, McCaffery PI)
  • Zeiss LSM 980 Airyscan 2 with PeCon cage incubator
  • Zeiss Axio Observer Yokogawa CSU-X1┬áspinning disk confocal equipped with dual Evolve EMCCDs, 405, 488, 561, and 633 nm lasers, FRAP module, and stage incubator for live imaging (acquired in part with NIH S10 award #1S10OD010712-01A1, McCaffery PI)
  • Nikon Sora Spinning Disk spinning disk confocal system with a super resolution mode, 7 laser lines and a fast CMOS camera.
  • Zeiss Axio Observer Epifluorescence SIM microscope equipped with Apotome 2 and Hamamatsu ORCA-Flash4.0 CMOS camera (acquired in part with NIH S10 award #1S10OD010712-01A1, PI McCaffery)
  • 3i Marianas Live Cell Imaging Workstation with In Vivo Scientific cage incubator equipped with dual Cascade II 512 EM cameras for simultaneous 2-channel acquisition and TIRF (acquired in part with NIH S10 award #1S10OD010712-01A1, McCaffery PI)
  • Zeiss Axiovert 200 LM with In Vivo Scientific cage incubator equipped with excitation and emission filter wheels and Cooke Sensicam camera; also equipped for microinjection and micromanipulation (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
  • Zeiss Axioplan II LM equipped with Cooke Sensicam digital monochrome camera and QImaging color camera (this system is excellent for polarized microscopy and color photomicrography.)
  • Zeiss Axio Zoom.V16 Fluorescence Stereo Zoom Microscope equipped with Axiocam MRm camera
  • Typhoon 9410 Variable Mode Imager capable of storage phosphor, chemiluminescence, and fluorescence (acquired with funds from NIH SIG award 1S10 RR021023-01, McCaffery PI)
  • Typhoon 5 Variable Mode Imager capable of storage phosphor, fluorescence, chemiluminescence, and densitometry (acquired via NIH SIG award 1 S10 OD021567-01A1, McCaffery PI

CELL SORTERS and FLOW CYTOMETERS:

3D Printing Core

MISCELLANEOUS/OTHER EQUIPMENT:

  • Avestin EmulsiFlex-C3 (acquired in part with funds from NIH R01GM060979-06, Wendland PI)
  • Data sever: 1.3 PB of storage managed by the IDIES, iic-data-01.idies.jhu.edu
  • Remote Linux, Mac, and PC image processing/analysis workstations including IMARIS