ELECTRON MICROSCOPES (and ancillary support equipment)

• FEI Talos 200SC FEG TEM equipped with Ceta and Falcon II Direct Detection CMOS cameras (NIH SIG award # 1S10RR023454-01, McCaffery PI)
• FEI Tecnai 12 TWIN TEM equipped with 16 bit 2K x2K FEI Eagle bottom mount camera; and SIS MegaView III wide-angle camera (acquired with funds from NSF DBI award # 0099706, McCaffery PI and NIH SIG award # 1S10OD012342-01A1, McCaffery PI)
• FEI Quanta 200 Environmental SEM (acquired with funds from HHMI undergraduate education grant)
• FEI Vitrobot vitrification robot (acquired with funds contributed by WSE and KSAS)
• Tousimis 795 Critical Point Dryer
• Anatech Hummer 6.02 Sputter Coater
• Denton 502A High Vacuum Evaporator
• Two Leica Ultracut UCT Ultramicrotomes/FCS Cryostages (acquired in part with funds from NSF DBI award # 0099706, McCaffery PI)

FLUORESCENCE MICROSCOPES (and related equipment):

• LaVision BioTec UltraMicroscope II light sheet microscope equipped with 405, 488, 561, 640, and 785 nm lasers and Okolab incubator (acquired with funds from NIH SIG award #1S10OD020152-01A1, McCaffery PI)
• Two Zeiss LSM 700 confocals (Left and Right) equipped with 405, 488, 555, and 633 nm lasers and PeCon cage incubators for live imaging
• Evident FV4000 confocal microscope with Picoquant FLIM/FCS attachment.
• Zeiss LSM 800 confocal with Airyscan super-resolution module, equipped with 405, 488, 561, and 633 nm lasers, motorized stages, and PeCon incubator(acquired with funds from NIH SIG award #1S10 OD020152-01A1, McCaffery PI)
• Zeiss LSM 980 Airyscan 2 with PeCon cage incubator
• Zeiss Axio Observer Yokogawa CSU-X1 spinning disk confocal equipped with dual Evolve EMCCDs, 405, 488, 561, and 633 nm lasers, FRAP module, and stage incubator for live imaging (acquired in part with NIH S10 award #1S10OD010712-01A1, McCaffery PI)
• Nikon SoRa Spinning Disk spinning disk confocal system with a super resolution mode, 7 laser lines and a fast CMOS camera.
• Zeiss Axio Observer Epifluorescence SIM microscope equipped with Apotome 2 and Hamamatsu ORCA-Flash4.0 CMOS camera (acquired in part with NIH S10 award #1S10OD010712-01A1, PI McCaffery)
• Zeiss Lattice Light Sheet microscope
• Zeiss Axiovert 200 LM with In Vivo Scientific cage incubator equipped with excitation and emission filter wheels and Cooke Sensicam camera; also equipped for microinjection and micromanipulation (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
• Zeiss Axioplan II LM equipped with Cooke Sensicam digital monochrome camera and QImaging color camera (this system is excellent for polarized microscopy and color photomicrography.)
• Zeiss Axio Zoom.V16 Fluorescence Stereo Zoom Microscope equipped with Axiocam MRm camera
• Typhoon 5 Variable Mode Imager capable of storage phosphor, fluorescence, chemiluminescence, and densitometry (acquired via NIH SIG award 1 S10 OD021567-01A1, McCaffery PI

CELL SORTERS and FLOW CYTOMETERS:

• Sony Biotechnology SH800 Cell Sorter (acquired with funds from NIH SIG award 1S10OD016230-01A1, McCaffery PI)
• Sony Biotechnology SY3200 Cell Sorter (acquired with funds from NIH SIG award 1S10OD016230-01A1, McCaffery PI)
• BD FACSCanto Flow Cytometer
• Beckman Coulter CytoFLEX LX Flow Cytometer (acquired with funds from NIH SIG award S10OD036395, Dobbie PI)
• FluoroFinder

3D Printing Core

• CELLINK BIO X6 Bioprinter with multiple printing, photo-curing, electro-magnetic droplet printheads
• CELLINK Lumen X photo-patterning instrument
• BMF-S140 High resolution 3D printer

MISCELLANEOUS/OTHER EQUIPMENT:

• Avestin EmulsiFlex-C3 (acquired in part with funds from NIH R01GM060979-06, Wendland PI)
• Data sever: 1.3 PB of storage managed by the IDIES, iic-data-01.idies.jhu.edu
• Remote Linux, Mac, and PC image processing/analysis workstations including IMARIS