Zeiss Apotome II system

Location: Dunning 106

A Zeiss Structured Illumination Microscopy system based on a Zeiss Axioobserver stand. This system allows rapid and low phototoxicity confocal like imaging. This system does not produce super resolution images.

Filter Sets:
Ex 640/30: dichroic 660 : Em 690/50. Cy5
Ex 550/25 : dichroic 570 : Em 605/70 HE DsRed
Ex 470/40 : dichroic 495 : Em 525/50 HE Green FP
Ex 365 : dichroic 395 : Em 445/50 DAPI
Yellow Apotome Calibration : unknown wavelengths
Analyser DIC transmitted light.

Illumination: Zeiss HXP metal halide light source

Camera: Hamamatzu Flash 4.0 sCMOS camera

Objectives:
2.5x 0.075NA
10x 0.3NA Ph1
20x 0.8NA
40x 1.3NA Oil
63x 1.4NA Oil
100x 1.4NA Oil DIC