Location: Dunning 106

Type: Inverted Nikon Ti2 with Yokogowa Sora Spinning Disk module

Lasers: 365 nm, 440 nm, 488 nm, 514 nm, 577 nm, 640 nm, 750 nm

Emission Filters:
445/49
481/36
531/50
547/20
606/18
647/100
691/64
823/100

Dichroics:
445/531/606/691/823 – penta bandpass
481/547/647 – tripple bandpass

Camera: Hamamatsu BT-Fusion

Objectives:
4x 0.1 NA
10x 0.45 NA
20x 0.8 NA
25 x 1.05 NA Silicone immersion oil (RI 1.406)
60x 1.42 Oil immersion
60 x 1.49 NA TIRF Oil immersion

Camera: Hamamatsu Orca Fusion-BT peak 95% QE, 2304 x 2304, 6.5 µm pixels.

Model fluorescence detection with SpekCheck

Note: The 577 nm laser means that Alexa 555 works very badly with this instrument, please use Alexa 568 instead.