Location: Croft 281

Type: FV4000 laser scanning confocal microscope with conventional and resonant scanner and Picoquant FLIM/FCS module

Lasers: 405 nm, 488 nm, 561 nm, 640 nm, 730 nm

Emission Filters: None, detection is fully spectrally determined.

Detectors: 4 spectral detectors: 2 Blue shifted, 2 red shifted and 1 transmission PMT.

PicoQuant addition: A full FLIM and FCS system with 440 nm, 470 nm and 640 nm pulsed lasers and 2 APD. There are a range of emission filters blocks for various collection approaches as well as the ability to collect orthogonal polarizations.

Objectives:
4x
10x 0.45 NA
20x 0.8 NA
40X SI 1.25 NA Silicone immersion (RI 1.4) with correction collar
60x 1.4 Oil immersion
60x 1.2 NA Water immersion with correction collar

Model fluorescence detection of PicoQuant config with SpekCheck

Model fluorescence detection with conventional confocal in SpekCheck