Evident FV4000 confocal

Location: Croft 281

Image of the FV4000 system

Type: FV4000 laser scanning confocal microscope with conventional and resonant scanner and Picoquant FLIM/FCS module

Lasers: 405 nm, 488 nm, 561 nm, 640 nm, 750 nm

Emission Filters: None, detection is fully spectrally determined.

Detectors: 4 spectral detectors: 2 Blue shifted, 2 red shifted and 1 transmission PMT.

PicoQuant addition: A full FLIM and FCS system with 440 nm, 470 nm and 640 nm pulsed lasers and 2 APD. There are a range of emission filters blocks for various collection approaches as well as the ability to collect orthogonal polarizations.

Objectives:
4x
10x 0.45 NA
20x 0.8 NA
40X SI 1.25 NA Silicone immersion (RI 1.4) with correction collar
60x 1.4 Oil immersion
60x 1.2 NA Water immersion with correction collar