![\"Waters](\"https:\/\/sites.krieger.jhu.edu\/mass-spec\/files\/2016\/02\/Waters-XevoG2.jpg\")
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The Waters UPLC-MS \u00a0system combines a high pressure (~15000 psi) Acquity H-Class UPLC system with a Xevo-G2 high mass resolution Q-ToF MS\/MS instrument. It is primarily used for specialist separation and analysis of biomolecules \u00a0such as metabolites, proteins and oligonucleotides. The LC includes an auto-sampler for automated multi sample analysis, the ability to blend up to four separate solvents streams, accommodation \u00a0for multiple columns, temperature controlled column compartments and a photodiode detector for UV spectroscopic detection of components to complement the mass spectral detection. The instrument is usually equipped with a selection of reverse phase high efficiency UPLC C4<\/sub> and C18<\/sub> columns and is supplied with Water, Methanol and Acetontrile solvents, along with a 0.1% Formic Acid modifier. The mass spectrometer is equipped with a dual ESI \/ APCI ion source and performs high resolution MS and MS\/MS analyses of chromatographic components in both positive and negative ionization modes. An internal lock-mass ensures high mass accuracy. Operation is by users on a \u201cself-service\u201d basis following familiarization and training and is largely automated using pre-defined methods that control instrument parameters such as column selection, column temperature, mobile phase composition and flow rate, mass spectral ionization conditions and mass range, etc. A separate work-station is provided for off-line data processing and includes specialist software for tasks such as structural identification of small molecules, sequencing and modification analysis of proteins and deconvolution of large multiply charged bio-molecules.<\/p>\n For information on access and user fees for this instrument, please contact the Facility Manager. The instrument is located in Room B14, Remsen Hall.<\/p>\n","protected":false},"excerpt":{"rendered":" The Waters UPLC-MS \u00a0system combines a high pressure (~15000 psi) Acquity H-Class UPLC system with a Xevo-G2 high mass resolution Q-ToF MS\/MS instrument. It is primarily used for specialist separation and analysis of biomolecules \u00a0such as metabolites, proteins and oligonucleotides. The LC includes an auto-sampler for automated multi sample analysis, the ability to blend up […]<\/p>\n","protected":false},"author":28,"featured_media":0,"parent":25,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-77","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/pages\/77","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/users\/28"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/comments?post=77"}],"version-history":[{"count":2,"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/pages\/77\/revisions"}],"predecessor-version":[{"id":98,"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/pages\/77\/revisions\/98"}],"up":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/pages\/25"}],"wp:attachment":[{"href":"https:\/\/sites.krieger.jhu.edu\/mass-spec\/wp-json\/wp\/v2\/media?parent=77"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}