{"id":625,"date":"2025-06-05T16:59:55","date_gmt":"2025-06-05T20:59:55","guid":{"rendered":"https:\/\/sites.krieger.jhu.edu\/iic\/?page_id=625"},"modified":"2026-03-25T15:01:15","modified_gmt":"2026-03-25T19:01:15","slug":"evident-fv4000-confocal","status":"publish","type":"page","link":"https:\/\/sites.krieger.jhu.edu\/iic\/instruments\/evident-fv4000-confocal\/","title":{"rendered":"Evident FV4000 confocal"},"content":{"rendered":"\n<p id=\"block-f6c5a8a3-4c15-470b-a79c-60cfbcbaf0fb\"><strong>Location:<\/strong> Croft 281<\/p>\n\n\n\n<figure class=\"wp-block-image alignright size-full is-resized\" id=\"block-4563171a-d098-458c-8f55-e0169a32c4bb\"><img decoding=\"async\" width=\"640\" height=\"641\" data-src=\"https:\/\/sites.krieger.jhu.edu\/iic\/files\/2025\/06\/FV4000-system-small.jpeg\" alt=\"Image of the FV4000 system\" class=\"wp-image-651 lazyload\" style=\"--smush-placeholder-width: 640px; --smush-placeholder-aspect-ratio: 640\/641;width:540px;height:auto\" data-srcset=\"https:\/\/sites.krieger.jhu.edu\/iic\/files\/2025\/06\/FV4000-system-small.jpeg 640w, https:\/\/sites.krieger.jhu.edu\/iic\/files\/2025\/06\/FV4000-system-small-300x300.jpeg 300w, https:\/\/sites.krieger.jhu.edu\/iic\/files\/2025\/06\/FV4000-system-small-150x150.jpeg 150w\" data-sizes=\"(max-width: 640px) 100vw, 640px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" \/><\/figure>\n\n\n\n<p id=\"block-17bef2e7-1e25-4b31-8829-6dd4f7e3be8f\"><strong>Type:<\/strong> FV4000 laser scanning confocal microscope with conventional and resonant scanner and Picoquant FLIM\/FCS module<\/p>\n\n\n\n<p id=\"block-d17e118f-7c9f-4f84-afcd-a1484b571847\"><strong>Lasers:<\/strong> 405 nm, 488 nm, 561 nm, 640 nm, 730 nm<\/p>\n\n\n\n<p id=\"block-231c7fb7-011d-4372-bd66-9605a1dc4ec7\"><strong>Emission Filters:<\/strong> None, detection is fully spectrally determined.<\/p>\n\n\n\n<p id=\"block-231c7fb7-011d-4372-bd66-9605a1dc4ec7\"><strong>Detectors<\/strong>: 4 spectral detectors: 2 Blue shifted, 2 red shifted and 1 transmission PMT.<strong> <\/strong><\/p>\n\n\n\n<p id=\"block-231c7fb7-011d-4372-bd66-9605a1dc4ec7\"><strong>PicoQuant addition:<\/strong> A full FLIM and FCS system with 440 nm, 470 nm and 640 nm pulsed lasers and 2 APD. There are a range of emission filters blocks for various collection approaches as well as the ability to collect orthogonal polarizations.<\/p>\n\n\n\n<p id=\"block-4476f3de-fb7d-4e99-a177-73f051176fee\"><strong>Objectives:<\/strong><br>4x<br>10x 0.45 NA<br>20x 0.8 NA<br>40X SI 1.25 NA Silicone immersion (RI 1.4) with correction collar<br>60x 1.4 Oil immersion<br>60x 1.2 NA Water immersion with correction collar<\/p>\n\n\n\n<p>Model fluorescence detection of PicoQuant config with <a href=\"http:\/\/iic-omero.win.ad.jhu.edu:8080\/?setup=Pico\">SpekCheck<\/a><\/p>\n\n\n\n<p>Model fluorescence detection with conventional confocal in <a href=\"http:\/\/iic-omero.win.ad.jhu.edu:8080\/?setup=FV4000\">SpekCheck<\/a><\/p>\n\n\n\n\n","protected":false},"excerpt":{"rendered":"<p>Location: Croft 281 Type: FV4000 laser scanning confocal microscope with conventional and resonant scanner and Picoquant FLIM\/FCS module Lasers: 405 nm, 488 nm, 561 nm, 640 nm, 730 nm Emission Filters: None, detection is fully spectrally determined. Detectors: 4 spectral detectors: 2 Blue shifted, 2 red shifted and 1 transmission PMT. PicoQuant addition: A full [&hellip;]<\/p>\n","protected":false},"author":389,"featured_media":0,"parent":222,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-625","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/625","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/users\/389"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/comments?post=625"}],"version-history":[{"count":4,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/625\/revisions"}],"predecessor-version":[{"id":714,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/625\/revisions\/714"}],"up":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/222"}],"wp:attachment":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/media?parent=625"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}