{"id":593,"date":"2024-04-18T14:04:42","date_gmt":"2024-04-18T18:04:42","guid":{"rendered":"https:\/\/sites.krieger.jhu.edu\/iic\/?page_id=593"},"modified":"2026-03-25T14:44:27","modified_gmt":"2026-03-25T18:44:27","slug":"nikon-sora-spinning-disk","status":"publish","type":"page","link":"https:\/\/sites.krieger.jhu.edu\/iic\/instruments\/nikon-sora-spinning-disk\/","title":{"rendered":"Nikon SoRa Spinning Disk"},"content":{"rendered":"\n

Location: <\/strong>Dunning 106<\/p>\n\n\n\n

\"Image<\/figure>\n\n\n\n

Type:<\/strong> Inverted Nikon Ti2 with Yokogowa Sora Spinning Disk module <\/p>\n\n\n\n

Lasers:<\/strong> 365 nm, 440 nm, 488 nm, 514 nm, 577 nm, 640 nm, 750 nm<\/p>\n\n\n\n

Emission Filters:<\/strong>
445\/49
481\/36
531\/50
547\/20
606\/18
647\/100
691\/64
823\/100<\/p>\n\n\n\n

Dichroics:<\/strong>
445\/531\/606\/691\/823 – penta bandpass
481\/547\/647 – tripple bandpass<\/p>\n\n\n\n

Camera:<\/strong> Hamamatsu BT-Fusion<\/p>\n\n\n\n

Objectives:<\/strong>
4x 0.1 NA
10x 0.45 NA
20x 0.8 NA
25 x 1.05 NA Silicone immersion oil (RI 1.406)
60x 1.42 Oil immersion
60 x 1.49 NA TIRF Oil immersion<\/p>\n\n\n\n

Camera:<\/strong> Hamamatsu Orca Fusion-BT peak 95% QE, 2304 x 2304, 6.5 \u00b5m pixels. <\/p>\n\n\n\n

Model fluorescence detection with SpekCheck<\/a><\/p>\n\n\n\n

Note: The 577 nm laser means that Alexa 555 works very badly with this instrument, please use Alexa 568 instead.<\/p>\n\n\n\n\n","protected":false},"excerpt":{"rendered":"

Location: Dunning 106 Type: Inverted Nikon Ti2 with Yokogowa Sora Spinning Disk module Lasers: 365 nm, 440 nm, 488 nm, 514 nm, 577 nm, 640 nm, 750 nm Emission Filters:445\/49481\/36531\/50547\/20606\/18647\/100691\/64823\/100 Dichroics:445\/531\/606\/691\/823 – penta bandpass481\/547\/647 – tripple bandpass Camera: Hamamatsu BT-Fusion Objectives:4x 0.1 NA 10x 0.45 NA20x 0.8 NA25 x 1.05 NA Silicone immersion oil (RI […]<\/p>\n","protected":false},"author":389,"featured_media":0,"parent":222,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-593","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/593","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/users\/389"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/comments?post=593"}],"version-history":[{"count":5,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/593\/revisions"}],"predecessor-version":[{"id":712,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/593\/revisions\/712"}],"up":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/222"}],"wp:attachment":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/media?parent=593"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}