{"id":393,"date":"2022-02-18T14:21:08","date_gmt":"2022-02-18T19:21:08","guid":{"rendered":"https:\/\/sites.krieger.jhu.edu\/iic\/?page_id=393"},"modified":"2026-01-08T14:34:23","modified_gmt":"2026-01-08T19:34:23","slug":"zeiss-axiozoom-16","status":"publish","type":"page","link":"https:\/\/sites.krieger.jhu.edu\/iic\/instruments\/zeiss-axiozoom-16\/","title":{"rendered":"Zeiss AxioZoom 16"},"content":{"rendered":"\n<figure class=\"wp-block-image alignright size-full is-resized\"><img decoding=\"async\" width=\"800\" height=\"624\" data-src=\"https:\/\/sites.krieger.jhu.edu\/iic\/files\/2023\/04\/image.png\" alt=\"Image of the AxioZoom 16 microscope\" class=\"wp-image-575 lazyload\" style=\"--smush-placeholder-width: 800px; --smush-placeholder-aspect-ratio: 800\/624;width:400px;height:312px\" data-srcset=\"https:\/\/sites.krieger.jhu.edu\/iic\/files\/2023\/04\/image.png 800w, https:\/\/sites.krieger.jhu.edu\/iic\/files\/2023\/04\/image-300x234.png 300w, https:\/\/sites.krieger.jhu.edu\/iic\/files\/2023\/04\/image-768x599.png 768w\" data-sizes=\"(max-width: 800px) 100vw, 800px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><strong>Location:<\/strong> Dunning Hall 103<\/p>\n\n\n\n<p><strong>Type:<\/strong> Upright zoom widefield system with transmission and fluorescence imaging <\/p>\n\n\n\n<p><strong>Illumination:<\/strong> Transmission with brightfield and  darkfield. Fluorescence with a Metal Halide lamp with DAPI,GFP and DsRed excitation filters, see filter cubes below<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-flow wp-block-group-is-layout-flow\">\n<p><strong>Emission channels: <\/strong>DAPI, GFP and DsRed<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-flow wp-block-group-is-layout-flow\">\n<p><strong>Filter Cubes:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>DAPI:      ex: 359\/48  dichroic: 395   em: 445\/50<\/li>\n\n\n\n<li>GFP:       ex: 470\/40  dichroic: 495   em: 525\/50<\/li>\n\n\n\n<li>DeRed:  ex: 550\/24.  dichroic: 570   em: 605\/70  <\/li>\n\n\n\n<li>Narrow GFP ex: 480\/20 dichroic: 495 em: 510\/20 (Chroma set 49020)<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\n<p><strong>Emission channels: <\/strong>DAPI, GFP and DsRed<\/p>\n\n\n\n<p><strong>Objectives: <\/strong><br>1x 0.25 NA<br>2.3x 0.57 NA<\/p>\n\n\n\n<p>Model fluorescence detection with <a href=\"http:\/\/iic-omero.win.ad.jhu.edu:8080\/?setup=AxioZoom\">SpekCheck<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Location: Dunning Hall 103 Type: Upright zoom widefield system with transmission and fluorescence imaging Illumination: Transmission with brightfield and darkfield. Fluorescence with a Metal Halide lamp with DAPI,GFP and DsRed excitation filters, see filter cubes below Emission channels: DAPI, GFP and DsRed Filter Cubes: Emission channels: DAPI, GFP and DsRed Objectives: 1x 0.25 NA2.3x 0.57 [&hellip;]<\/p>\n","protected":false},"author":389,"featured_media":0,"parent":222,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-393","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/393","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/users\/389"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/comments?post=393"}],"version-history":[{"count":5,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/393\/revisions"}],"predecessor-version":[{"id":692,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/393\/revisions\/692"}],"up":[{"embeddable":true,"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/pages\/222"}],"wp:attachment":[{"href":"https:\/\/sites.krieger.jhu.edu\/iic\/wp-json\/wp\/v2\/media?parent=393"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}