Lecomte Research Group > People > Christopher Falzone

Christopher Falzone

Christopher J. Falzone

Department of Chemistry
Johns Hopkins University
314 New Chemistry Building
3400 N. Charles St.
Baltimore, MD 21218
Phone: (410) 516-7467
Fax: (410) 516-8420
email: falzone@jhu.edu

Education

  • A. B. in Chemistry, Washington University (St. Louis)
  • Ph. D. in Chemistry, 1984, Clarkson University (Potsdam, NY)
  • Post-Doctoral Associate, University of Akron
  • Post-Doctoral Associate, The Pennsylvania State University

Professional Experience

  • 1986–1987: Director of Nuclear Magnetic Resonance Instrumentation, The University of Akron
  • 1992–present: Lecturer, The Pennsylvania State University
  • 1992–present: Assistant Director of the Nuclear Magnetic Resonance Facility (Biological Applications), The Pennsylvania State University
  • 1997–present: Chemistry Co-op Coordinator, The Pennsylvania State University
  • 1997–present: Chemistry Undergraduate Advising Coordinator, The Pennsylvania State University
  • 2003–2007: Teaching Professor, Chemistry Department, The Pennsylvania State University
  • 2007–present: Associate Research Professor, Chemistry Department, Johns Hopkins University

Publications

  • Antonkine, M.L., Maes, E.M., Czernuszewicz, R.S., Breitenstein, C., Bill, E., Falzone, C.J., Balasubramanian, R., Lubner, C., Bryant, D.A., and Golbeck, J.H. (2007) Chemical rescue of a site-modified ligand to a [4Fe-4S] cluster in PsaC, a bacterial-like dicluster ferredoxin bound to Photosystem I. Biochimica Biophysica Acta 1767:712–724
  • Vuletich, D.A., Falzone, C.J., and Lecomte, J.T.J. (2006) Structural and Dynamic Repercussions of Heme Binding and Heme-Protein Cross-Linking in Synechococcus sp. PCC 7002 Hemoglobin. Biochemistry 45:14075–14084.
  • Lecomte, J.T.J., Vu, B.C., and Falzone,C.J. (2005) Structural and Dynamic Properties of Synechocystis sp. PCC 6803 Hb Revealed by Reconstitution with Zn-Protoporphyrin IX. Journal of Inorganic Biochemistry 99:1585–1592.
  • Vu, B.C., Nothnagel, H.J., Vuletich, D.A., Falzone, C.J., and Lecomte, J.T.J. (2004) Cyanide Binding to Hexacoordinate Cyanobacterial Hemoglobins: Hydrogen Bonding Network and Heme Pocket Rearrangement in Ferric H117A Synechocystis Hb. Biochemistry, 43:12622–12633. 
  • Vu, B.C., Vuletich, D.A., Kuriakose, S.A., Falzone, C.J., and Lecomte, J.T.J. (2004) Characterization of the Heme-Histidine Cross-link in Cyanobacterial Hemoglobins from Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002. Journal of Biological Inorganic Chemistry 9:183–194.
  • Vadrevu, R., Falzone, C.J., and Matthews, C. R. (2003) Partial NMR Assignments and Secondary Structure Mapping of the Isolated a-Subunit of Escherichia coli Tryptophan Synthase, a 29-kD TIM Barrel Protein. Protein Science12:185–191.
  • Proctor, D.J., Schaak, J.E., Bevilacqua, J.M., Falzone C.J., & Bevilacqua, P.C. (2002) Isolation and Characterization of a Family of Stable RNA Tetraloops with the Motif YNMG That Participate in Tertiary Interactions. Biochemistry, 41:12062–12075.
  • Falzone, C.J., Vu, B.C., Scott, N.L., and Lecomte, J.T.J. (2002) The Solution Structure of the Recombinant Hemoglobin from the Cyanobacterium Synechocystis sp. PCC 6803 in its Hemichrome State. Journal of Molecular Biology 324:1015–1029.
  • Scott, N.L., Falzone, C.J., Vuletich, D.A., Zhao, J., Bryant, D.A., and Lecomte, J.T.J. (2002) Truncated Hemoglobin from the Cyanobacterium Synechococcus sp. PCC 7002: Evidence for Hexacoordination and Covalent Adduct Formation in the Ferric Recombinant Protein. Biochemistry 22:6902–6910. 
  • Falzone, C.J., and Lecomte, J.T.J. (2002) Letter to the Editor: Assignment of the 1H, 13C, and 15N Signals of Synechocystis sp. PCC 6803 Methemoglobin. Journal of Biomolecular NMR 23:71–72. 
  • Lecomte, J.T.J., Scott, N.L., Vu, B.C., and Falzone, C.J. (2001) Binding of Ferric Heme by the Recombinant Globin from the Cyanobacterium Synechocystis sp. PCC 6803. Biochemistry 40:6541–6552. 
  • Falzone, C.J., Wang, Y., Vu, B.C., Scott, N.L., Bhattacharya, S., and Lecomte, J.T.J. (2001) Structural and Dynamic Perturbations Induced by Heme Binding in Cytochrome b5. Biochemistry 40:4879–4891. 
  • Lecomte, J.T.J., and Falzone, C.J. (1999) Where U and I meet. Nature Structural Biology, 6:605-608.
  • Mayer, K.L., Shen, G., Bryant, D.A., Lecomte, J.T.J., and Falzone, C.J. (1999) The Solution Structure of Photosystem I Accessory Protein E from the Cyanobacterium Nostoc sp. Strain PCC 8009. Biochemistry 38:13736–13746.
  • Lecomte, J.T.J., Sukits, S.F., Bhattacharya, S., and Falzone, C.J. (1999) Conformational Properties of Native Sperm Whale Apomyoglobin in Solution. Protein Science 8:1484–1491.
  • Bhattacharya, S., Falzone, C.J., and Lecomte, J.T.J. (1999) The Backbone Dynamics of Apocytochrome b5 in Its Native, Partially Folded State. Biochemistry 38:2577–2589. doi:10.1021/bi995081u
  • Falzone, C.J., Mayer, M.R., Whiteman, E.L., Moore, C.D., and Lecomte, J.T.J. (1996) Design Challenges for Hemoproteins: The Solution Structure of Apocytochrome b5. Biochemistry 35:6519–6526. 
  • Falzone, C.J., Kao, Y.-H., Zhao, J., Bryant, D.A., and Lecomte, J.T.J. (1994) Three-Dimensional Solution Structure of PsaE from the Cyanobacterium Synechococcus sp. Strain PCC 7002: a Photosystem I Protein that Shows Structural Homology with SH3 Domains. Biochemistry 33:6052–6062. Protein data bank id: 
  • Falzone, C.J., Kao, Y.-H., Zhao, J., MacLaughlin, K.L., Bryant, D.A., and Lecomte, J.T.J. (1994) 1H and 15N NMR Assignments of PsaE, a Photosystem I Subunit from the Cyanobacterium Synechococcus sp. Strain PCC 7002. Biochemistry 33:6043–6051.
  • Falzone, C.J. Wright, P.E. and Benkovic, S.J. (1994) Dynamics of a Flexible Loop in Dihydrofolate Reductase from Escherichia coli by Site-directed Mutagenesis. Biochemistry 33:439-442.
  • Falzone, C.J., Cavanagh, J., Cowart, M., Palmer, A.G. III, Matthews, C. R., Benkovic, S.J. and Wright, P.E. (1994) The 1H, 15N, and 13C Resonance Assignments, Secondary Structure, and the Conformation of Substrate in the Binary Folate Complex of Escherichia coli Dihydrofolate Reductase. Journal of Biomolecular NMR 4:349-366.
  • Cowart, M., Falzone, C. J., and Benkovic, S. J. (1994) A New Synthesis of Double Labeled [7,9– 13C2] Folic Acid. Journal of Labelled Compounds and Radio-pharmaceuticals 34:67–71.
  • Benesi, A., J., Falzone, C. J., Banerjee, S., and Farber, G. K. (1994) NMR Assignments for the Aldopentoses. Carbohydrate Research 258:27–33.
  • Iwakura, M., Jones, B. E., Falzone, C. J., and Matthews, C. R. (1993) Collapse of Parallel Folding Channels in Dihydrofolate Reductase from Escherichia coli by Site–Directed Mutagenesis. Biochemistry 32:13566–13574.
  • Li, L., Falzone, C. J., Wright, P. E., and Benkovic, S. J. (1992) Functional Role of a Mobile Loop of Escherichia coli Dihydrofolate Reductase in Transition State Stabilization. Biochemistry 31:7826–7833.
  • Falzone, C.J., Benesi, A.J., and Lecomte, J.T.J. (1992) Characterization of Taxol in Methylene Chloride by NMR Spectroscopy. Tetrahedron Letters 33:1169–1172.
  • Falzone, C. J., Wright, P. E., and Benkovic, S. J. (1991) Evidence for Two Interconverting Protein Isomers in the Methotrexate Complex of Dihydrofolate Reductase from Escherichia coli. Biochemistry 30:2184–2191.
  • Wilson, G. E., Falzone, C. J., and Dong, H. (1990) GDP and GTP Association Equilibria. Use of Manganous Ion Induced Paramagnetic Relaxation Rates to Study Hydrogen–Bonded Species. Journal of the American Chemical Society 112:8269–8273.
  • Falzone, C. J., Benkovic, S. J., and Wright, P. E. (1990) Partial 1H NMR Assignments of the Escherichia coli Dihydrofolate Reductase Complex with Folate: Evidence for a Unique Conformation of Bound Folate. Biochemistry 29: 9667–9677.
  • Falzone, C. J., Karsten, W. E., Conley, J. D., and Viola, R. E., (1988) L–Aspartase from Escherichia coli: Substrate Specificity and the Role of Divalent Metal Ions. Biochemistry 27:9089–9093.
  • Guterman, L. R., Falzone, C. J., Wilson, G. E., Jr. (1986) Action of Hydrochloric Acid on Aluminum Hydroxide-Magnesium Hydroxide Gels and Magaldrate: Quasi-elastic Light Scattering Studies. Journal of Pharmaceutical Sciences 75:502–505.
  • Wilson, G. E., Jr., Falzone, C. J., Lee, H. K., and Johnson, R. (1985) The Action of Hydrochloric Acid on Aluminum Hydroxide–Magnesium Hydroxide Gels and Magaldrate: 27Al NMR and pH-stat Studies. Journal of Pharmaceutical Sciences 74:1075–1077.