Structural and Dynamic Characterization of 2/2 Hemoglobins
Funded by the National Science Foundation
Several unicellular photosynthetic organisms are known to produce one or more hemoglobins. The properties and function of these proteins remain mysteries that offer a window on heme chemistry and molecular evolution. The goal of the project is to study the relationship between structure and reactivity in the hemoglobins of Synechocystis, Synechococcus, and Chlamydomonas. NMR methods are applied to determine the structure and dynamics of the proteins and to identify the residues participating in the control of ligand binding. Because of the ancient origin of the hemoglobin superfamily, the properties of these hemoglobins under extreme conditions of pressure are also a focus of investigation.